The Mary Lyon Centre at the Medical Research Council Harwell is recruiting a Research Assistant on the MLC Graduate Scheme to its cell culture laboratories, to provide technical support in a busy molecular biology service team. Their activity will span a range of molecular biology and cell culture techniques, including learning to culture both mouse ES cell and organoid lines. Additional training in a range of soft skills will be provided as a part of a Graduate cohort undergoing technical training in a range of specialities.
This Graduate placement is based within a long-established laboratory tasked with the generation and quality control of ES cell and organoid lines leading to the generation of genetically defined mouse lines at the Mary Lyon Centre. Youwill gain highly coveted expertise in the culture of ES cells and organoids and will be taught to implement a range of molecular protocols (preparation of DNA, PCR, digital PCR, CRISPR-guide cloning and transfection) in a fast-paced environment with a strong team focus. Emphasis on particular techniques will vary according to service demand. In addition, the graduate will gain a deep understanding of the many different types of genetic alterations that are employed in laboratory animals.
This represents a unique opportunity to acquire a strong practical knowledge in a broad range of highly desirable cellular and molecular biology skills in a group recognised for its expertise and the quality of its output.
Additional training in a range of soft skills will be provided as part of a Graduate cohort undergoing technical training in a range of specialities.
Main duties/key responsibilities
- The culture of mouse embryonic fibroblast cells, stem cells and organoid lines.
- Using a variety of electroporation techniques to develop novel ES cell lines with known genetic modifications and the quality control of those lines.
- Preparation of ES cells and organoid cell lines for micro-injection to generate mouse models.
- Sample management and communication of results internally.
- Other general duties required are to ensure smooth running of a cellular biology laboratory i.e. ordering reagents – tasks shared with the rest of the team.
Working relationships
Working as part of a team with the other cell culture team members, Develop and culture ES cell and organoid lines as directed by the cell culture laboratory manager.
Work with a range of technical support staff, including highly trained post-doctoral technologists, to produce cellular and molecular biology work for the molecular and cellular biology group for the Mary Lyon Centre.
Additional information
Some flexible work patterns and paid weekend work may be required at the discretion of the supervisor and as part of a team rota.
Person specification
Education/qualifications/training required
Essential:
- BSc in Biological Sciences or related.
- Understanding of basic molecular techniques (PCR, qPCR)
- Understanding of basic cell culture techniques (media preparation, sterile conditions)
Desirable:
- Understanding of genome engineering techniques (gene targeting, CRISPR-based tools)
Previous work experience required
Essential:
- Work in a laboratory.
Desirable:
- Previous work with genetically modified materials.
- Evidence of quality focused work.
Knowledge and experience
Essential:
- Some initial experience in basic techniques of Molecular Biology e.g. PCR, cloning, or qPCR.
- Computer literacy is essential for the recording and communication of results.
- Some experience of work in sterile conditions.
Desirable:
- An understanding of genetic modification in laboratory animals.
Personal skills/behaviours/qualities
Essential:
- Organised, hard-working with precision and a strong eye for detail.
- Able to work well as part of a team, as well as independently.
- Good communication skills, both written and verbal.
Further information
Equality & Diversity
The MRC values the diverse skills and experience of its employees and is committed to achieving equality of treatment for all. Our objectives are that all individuals shall have equal opportunities for employment and advancement on the basis of their skills, aptitudes and abilities. The MRC is committed to the engagement and retention of the best possible talent and to creating an environment that encourages excellence in scientific research through good equality and diversity leadership and management.
Corporate/Local Responsibilities & Requirements
The post-holder must at all times carry out their responsibilities with due regard to the MRC’s:
- Code of Conduct
- Equality and Diversity policy
- Health and Safety policy
- Data Protection and Security policy
Job descriptions should be reviewed on a regular basis and at the annual appraisal. Any changes should be made and agreed between the post-holder and their manager.
The above lists are not exhaustive and the post-holder is required to undertake such duties as may reasonably be requested within the scope of the post. All employees are required to act professionally, cooperatively and flexibly in line with the requirements of the post and the MRC.
